Thermo Fisher Scientific Phospho-Rho Kinase/ROCKII (Thr396) Polyclonal Antibody
Phospho-Rho Kinase/ROCKII (Thr396) 특이적 폴리클로날 항체로, WB 및 IHC(P) 실험에 적합. Rabbit IgG 호스트 기반, 항원 친화 크로마토그래피로 정제. ROCK2 단백질의 Thr396 인산화 부위 인식. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the epitope VETFP with a single phosphorylation site Thr396 of human RockII protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Positive control: Conditioned 3T3 cells
Target Information
ROCK2, the predominant ROCK isoform in skeletal muscle, is an important regulator downstream of Rho GTPases.
ROCK2 regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element.
This protein, which is an isozyme of ROCK1, is a target for the small GTPase Rho.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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