Thermo Fisher Scientific EHMT1 Polyclonal Antibody
EHMT1 단백질을 인식하는 Rabbit Polyclonal 항체로 Western Blot, IHC, IP 등 다양한 응용에 적합. 액상 형태로 제공되며 4°C에서 보관. FFPE 조직의 IHC 시 citrate buffer(pH 6.0) 항원 회복 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 25 and 75 of human euchromatic histone-lysine N-methyltransferase 1 (EHMT1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
EHMT1 (also known as EUHMTASE1) is a histone methyltransferase that methylates Lys-9 of histone H3 in vitro.
H3 Lys-9 methylation acts as an epigenetic mark for transcriptional repression by recruiting HP1 proteins to methylated histones.
EHMT1 is likely targeted to histone H3 by DNA-binding proteins such as E2F6, MGA, MAX, and/or DP1.
During the G0 phase, it may contribute to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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