Thermo Fisher Scientific CD100 Monoclonal Antibody (A8), PE
Flow Cytometry용 CD100 (Semaphorin 4D) 단클론 항체로, PE 형광 표지된 Mouse IgG1 형식입니다. 인간 PHA 활성화 림프구를 면역원으로 제작되었으며, 565/576 nm 파장에서 Excitation/Emission 특성을 가집니다. 4°C 암조건 보관, 동결 금지.
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Applications
- Flow Cytometry (Flow): Neat
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A8 |
| Immunogen | Human PHA activated lymphocytes |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2538063 |
Product Specific Information
- Reconstitute with 1 mL of distilled water.
- After reconstitution, store product undiluted at 4°C in the dark.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Target Information
Semaphorins are neuronal chemorepellents that guide pioneering neurons during nervous system development.
CD100 (Semaphorin 4D) is a 150 kDa homodimer that induces B cell aggregation and enhances their viability in vitro.
It modulates CD40-CD40L B cell signaling by promoting aggregation, increasing survival, and downregulating CD23 expression.
CD72 serves as a receptor for CD100, regulating B cell signaling.
CD100 is expressed on resting and PHA-stimulated T cells, weakly on NK cells, EBV-transformed B cells, monocytes, and tumor/peripheral blood T cell lines, and more strongly on activated T and B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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