Thermo Fisher Scientific Glycerol Kinase Polyclonal Antibody, HRP
Western blot, ELISA, Immunoprecipitation에 적합한 HRP 결합 다클론 항체. Cellulomonas species 유래 Glycerol Kinase 인식. 염교환 크로마토그래피로 정제된 고순도 항체. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,500 |
| ELISA | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 1:500–1:2,500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Glycerol Kinase (Cellulomonas species) |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Glycerol Kinase antibody is an IgG fraction purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, and purified or partially purified Glycerol Kinase (Cellulomonas species).
Cross-reactivity with Glycerol Kinase from other sources is unknown.
Target Information
Glycerol kinase belongs to the FGGY kinase family and catalyzes the phosphorylation of glycerol by ATP to produce ADP and glycerol-3-phosphate.
It plays a critical role in glycerol uptake and metabolism.
Defects in this gene cause glycerol kinase deficiency (GKD).
Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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