Thermo Fisher Scientific Glycerol-3-Phosphate Dehydrogenase Polyclonal Antibody, HRP

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,500

ELISA

• Tested Dilution: 1:1,000–1:5,000

Immunoprecipitation (IP)

• Tested Dilution: 1:500–1:2,500

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Glycerol-3-Phosphate Dehydrogenase antibody is purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, ion-exchange chromatography, and dialysis against the specified buffer.
Immunoelectrophoresis shows a single precipitin arc against anti-Peroxidase, anti-Goat Serum, and purified Glycerol-3-Phosphate-Dehydrogenase (Rabbit Muscle).
Cross-reactivity with Glycerol-3-Phosphate-Dehydrogenase from other sources is unknown.

Target Information

This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The protein catalyzes the reversible conversion of dihydroxyacetone phosphate (DHAP) and NADH to glycerol-3-phosphate (G3P) and NAD+, playing a key role in carbohydrate and lipid metabolism.
Cytosolic and mitochondrial forms form a glycerol phosphate shuttle transferring reducing equivalents from cytosol to mitochondria.
Mutations cause transient infantile hypertriglyceridemia.
Multiple alternatively spliced transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.