Thermo Fisher Scientific Phospho-HMGN (Ser20, Ser24) Polyclonal Antibody
인간 HMGN 단백질의 Ser20 및 Ser24 인산화 잔기를 인식하는 폴리클로날 항체입니다. Western blot, IHC, ELISA에 적합하며 높은 특이성과 재현성을 제공합니다. Affinity chromatography로 정제되어 안정적인 액상 형태로 제공됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 20 µg/mL |
| ELISA | 1:5,000–1:25,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 19–28 of human HMGN protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. Stable for several weeks at 4°C as undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against phosphorylated human HMGN protein at pS20 and pS24 residues. Reactivity occurs against human HMGN pS20/pS24 proteins and is specific for the phosphorylated form. Reactivity is seen against HMGN1 and HMGN2; HMGN3 is likely reactive but not tested. Reactivity with non-phosphorylated HMGNs is minimal by ELISA. BLAST analysis suggests cross-reactivity with HMGN proteins from Xenopus, chicken, mouse, bovine, dog, orangutan, rat, and swine based on 100% homology with the immunizing sequence.
Target Information
The high-mobility group (HMG) proteins 14 and 17 are abundant chromosomal proteins that bind to nucleosomes and enhance transcription. They act as architectural elements altering chromatin structure and facilitating transcription from chromatin templates. HMG-14/17 proteins modify nucleosomal organization and mediate unfolding of higher-order chromatin structure, providing DNA accessibility. These proteins are also constitutive components of mouse oocyte and embryonic chromatin, linking chromatin structure to embryonic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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