Thermo Fisher Scientific Click-iT AHA Alexa Fluor 488 Protein Synthesis HCS Assay

Invitrogen™ Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay

The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic, and non-radioactive method for detecting nascent protein synthesis using fluorescence microscopy and high-content imaging.

Click-iT® AHA is an amino acid analog of methionine containing an azido moiety. Similar to ³⁵S-methionine, Click-iT® AHA is fed to cultured cells and incorporated into proteins during active protein synthesis. Detection of the incorporated amino acid utilizes a chemoselective ligation or “click” reaction between an azide and an alkyne, where the azido-modified protein is detected with the green-fluorescent Alexa Fluor® 488 alkyne.

This assay has been successfully tested in A549 and U-2 OS cells with various protein synthesis inhibitors, including cycloheximide, anisomycin, and puromycin, in both dose-response and Min/Max formats.

주요 특징

• 비방사성, 무독성 단백질 합성 검출
• 고감도 형광 기반 분석
• HCS 및 형광 현미경 장비와 호환
• 다양한 억제제와 검증 완료

사양

제품 이미지