Invitrogen™

Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay

The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the자세히 알아보기

The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging. Click-iT® AHA is an amino acid analog of methionine containing an azido moiety. Similar to 35S-methionine, Click-iT® AHA is fed to cultured cells and incorporated into proteins during active protein synthesis. Detection of the incorporated amine acid utilizes a chemoselectiove ligation or “click` reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor® 488 alkyne. The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min⁄Max format.

사양

색상Blue, Green

검출 방법Fluorescence

용도(애플리케이션)High-content Screening (HCS)

용도 (장비)Fluorescence Microscope, High Content Analysis Instrument

제품 유형HCS Assay

염료 유형Alexa Fluor™ 488, Hoechst 33258

방출UV, Visible

형식96-well plate

그린 기능Less hazardous

제품라인Alexa Fluor™, Click-iT™, Molecular Probes™

수량2 plates

배송 조건Room Temperature

Unit SizeEach