Thermo Fisher Scientific Click-iT RNA Alexa Fluor 488 Imaging Kit

Invitrogen™ Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit

The Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit enables detection of global RNA transcription temporally and spatially in cells and tissues (PNAS (2008) 105:15779-84).
This kit allows researchers to visualize newly synthesized RNA or detect changes in RNA levels resulting from disease, environmental damage, or drug treatments—an important aspect of toxicological profiling.

Utilizing an alkyne-modified nucleoside, 5-ethynyl uridine (EU), and powerful click chemistry, newly synthesized RNA can be detected without the use of radioactivity or antibodies through a simple two-step procedure:

1. Incorporation step: The alkyne-containing nucleoside is fed to cells or animals and actively incorporated into nascent RNA. The small size of the tag enables efficient incorporation into RNA, but not into DNA.
2. Detection step: Detection utilizes the chemoselective ligation (“click” reaction) between an azide and an alkyne, where the modified RNA is detected with an azide-containing dye.

With its diminutive footprint, the Click-iT™ detection molecule can easily penetrate complex samples and allows multiplex analyses with other probes, including antibodies for detecting RNA-interactive proteins, enabling deeper biological insights.

사양 (Specifications)

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