Invitrogen™

Click-IT™ Palmitic Acid, Azide (15-Azidopentadecanoic Acid)

Identify and characterize palmitylated proteins with Click-iT® palmitic acid, azide using the powerful click chemistry, a simple and robust two-step자세히 알아보기

Identify and characterize palmitylated proteins with Click-iT® palmitic acid, azide using the powerful click chemistry, a simple and robust two-step labeling and detection technique. In step one, the azide-containing biomolecule is fed to cells or animals and actively incorporated into proteins. Unlike other labels such as biotin or a fluorescent dye, the azide-tag is small enough that the tagged molecule is an acceptable substrate for the enzymes that incorporate this building block into proteins. Detection utilizes the chemoselective ligation or “click` reaction between and azide and an alkyne where the modified protein is detected with the corresponding alkyne-containing dye or hapten using either the Click-iT® Cell Reaction Buffer Kit or the Click-iT® Protein Buffer Kit. With the Click-iT® Cell Reaction Buffer Kit, cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis (HCS) together with other biomarkers of interest for content and context rich results. With the Click-iT® Protein Reaction Buffer Kit, achieve detection sensitivity in 1-D gels and western blots in the low femtomole range or perform LC-MS⁄MS and MALDI MS analysis.

사양

그린 기능Less hazardous

제품 유형Palmitic Acid

수량1 mg

라벨링 방법Metabolic Labeling

배송 조건Room Temperature

제품라인Click-iT™, Molecular Probes™

라벨 또는 염료Azide

형식Solid

Labeling TargetProteins, Proteins, Proteins

Unit SizeEach