Thermo Fisher Scientific Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker) Monoclonal Antibody (SPM528)
Ep-CAM/CD326 단백질을 인식하는 인간 특이적 단일클론 항체로, 다양한 면역분석(WB, IHC, ICC, Flow)에 사용 가능. 단백질 A/G 정제, 액상 형태, 4°C 보관. Ep-CAM 발현 암세포 및 상피세포 마커 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution / Conditions |
|---|---|
| Western Blot (WB) | 1:100–1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 5–10 µL / 10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM528 |
| Immunogen | Neuraminidase-treated GLS-1 human small cell lung carcinoma cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Rat.
Target Information
Ep-CAM (epithelial adhesion molecule, also known as epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues.
It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism through induction of proto-oncogene c-myc and cyclins A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, impacting epithelial differentiation and growth.
Overexpression of Ep-CAM is associated with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors.
Ep-CAM is typically localized on the basolateral surface of simple epithelia and various carcinoma types, and is useful in distinguishing adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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