Thermo Fisher Scientific Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker) Monoclonal Antibody (SPM528)
Ep-CAM/CD326 단백질을 인식하는 마우스 단클론 항체로, 인간 시료에 반응합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. 단백질 A/G로 정제된 액상 형태이며 보존제 무첨가로 -20°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 5–10 µL / 10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM528 |
| Immunogen | Neuraminidase-treated GLS-1 human small cell lung carcinoma cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Product Specific Information
- Does not react with Rat.
Target Information
Ep-CAM (epithelial adhesion molecule, also known as epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues.
It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism by regulating genes such as c-myc, cyclin A, and cyclin E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth.
Overexpression of Ep-CAM is associated with enhanced epithelial proliferation and is a known marker for epithelial-derived tumors.
It is expressed on the basolateral surface of most simple epithelia and many carcinoma types, and can distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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