Thermo Fisher Scientific TCR alpha/beta Monoclonal Antibody (IP26), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (1 µg)/test
• Publications: View 2 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Red 710
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine5
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The IP26 monoclonal antibody reacts with the human alpha beta TCR dimer, which is expressed by the majority of peripheral T cells.

Applications Reported:
This antibody is reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (1 µg) per test (final volume 100 µL). Cell number range: 10⁵–10⁸ cells/test.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and compensation.
Staining may be obstructed when co-stained with OKT3 (anti-human CD3).

Excitation/Emission: 488–561 nm / 775 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The T cell antigen receptor (TCR) is a ligand-specific alpha/beta heterodimer associated with invariant CD3 chains (gamma, delta, eta, zeta). These subunits are essential for surface expression and signal transduction. TCR engagement triggers T cell activation, differentiation, and cytokine production.

Most T cells express TCR alpha/beta with CD4 or CD8 (single positive). A small subset lacks both (double negative), which can increase in autoimmune or immunodeficiency disorders. Gamma-delta T cells, found mainly in epithelial tissues, have distinct antigen recognition and may exhibit antitumor or immunoregulatory roles.

TCR recognition of self-peptides is linked to autoimmune disease, while mutant self-peptides are associated with tumors. Superantigen engagement of TCRs can lead to toxic shock syndrome. Autoantibodies to TCR V beta segments have been isolated from patients with rheumatoid arthritis and systemic lupus erythematosus, potentially modulating immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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