Thermo Fisher Scientific GFP Monoclonal Antibody (9F9.F9)

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• AP
• Biotin
• DyLight 405, 488, 549, 649, 680, 800
• FITC
• HRP
• Rhodamine
• Custom conjugation available upon request

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot and freeze at -20°C or below for extended storage.
• Avoid repeated freeze/thaw cycles.
• Centrifuge if not clear after standing at room temperature.
• Stable for several weeks at 4°C as undiluted liquid.
• Dilute only immediately before use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant GFP from Aequorea victoria by both Western blot and ELISA. No cross-reactivity with RFP was observed.

Target Information

The jellyfish Aequorea victoria produces Green Fluorescent Protein (GFP), which emits green light during bioluminescence. GFP is widely used as a reporter protein in both eukaryotic and prokaryotic systems and as a tag for protein localization studies. It enables visualization of protein movement and subcellular dynamics. GFP is a 27 kDa monomeric protein that forms its fluorescent chromophore autocatalytically, absorbing at 395 nm and emitting at 508 nm. Its fluorescence is stable and species-independent, suitable for diverse applications including gene expression monitoring, protein localization, protein–protein interaction studies (e.g., yeast two-hybrid), and FRET experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.