Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Red 725, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Red 725, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Species Reactivity

Human, Non-human primate

Product Specifications

Additional Formats

• APC-eFluor 780
• Functional Grade
• NovaFluor Blue 510 / 530 / 585 / 610-70S / 660-120S / 660-40S / 760
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 590 / 610 / 660 / 690 / 700 / 730 / 755
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702
• Request custom conjugation available

Product Specific Information

This 3G8 monoclonal antibody reacts with human and non-human primate CD16 (FcγRIII), which exists as FcγRIIIA and FcγRIIIB isoforms.
FcγRIIIA is expressed on monocytes, macrophages, and NK cells, while FcγRIIIB is found on neutrophils. Expression patterns differ among primates.

Binding of IgG to CD16 triggers signal transduction leading to ADCC, phagocytosis, cytokine release, and antigen presentation.
Clone 3G8 recognizes the same epitope as CB16 but differs from B73.1.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested for flow cytometry using normal human peripheral blood cells. Recommended usage: 5 µL (0.6 µg) per test in 100 µL final volume.

Note: NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.

CellBlox Plus Blocking Buffer:
Contains formulation improvements over CellBlox. Add 5 µL per sample for optimal staining. For single-color controls, use 5 µL per 100 µL of cell sample.

NovaFluor Technology:
Based on Phiton™ nucleic acid dye structures engineered for optimized fluorescence with minimized spillover and spread.

Excitation: 636 nm
Emission: 727 nm
Laser: 633–640 nm (Red)

Target Information

CD16 encodes an Fc receptor (FcγRIII) that binds the Fc portion of IgG, mediating immune complex clearance and ADCC.
Gene FCGR3A (chromosome 1) is expressed on NK cells, while FCGR3B is expressed on neutrophils.
Mutations in FCGR3A are linked to immunodeficiency 20, viral infection susceptibility, systemic lupus erythematosus, and neonatal neutropenia.
Alternative splicing produces multiple isoforms.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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