Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Red 755, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Red 755, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• APC-eFluor 780
• Functional Grade
• NovaFluor Blue 510 / 530 / 585 / 610-70S / 660-120S / 660-40S / 760
• NovaFluor Red 685 / 700 / 725
• NovaFluor Yellow 590 / 610 / 660 / 690 / 700 / 730 / 755
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702
• Request custom conjugation available

Product Specific Information

This 3G8 monoclonal antibody reacts with human and non-human primate CD16 (low-affinity Fc gamma RIII). CD16 exists as two isoforms, FcγRIIIA and FcγRIIIB.

• FcγRIIIA: Expressed on monocytes, macrophages, NK cells
• FcγRIIIB: Detected on neutrophils (GPI-anchored form)

In non-human primates, CD16 expression patterns vary by species. Binding of IgG activates signal transduction leading to ADCC, phagocytosis, cytokine release, and antigen presentation.

Based on cross-blocking studies, clone 3G8 recognizes the same epitope as CB16 but distinct from B73.1.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry on human peripheral blood cells.
Use 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample (regardless of NovaFluor count).
  • For single-color controls: 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates are based on Phiton™ technology utilizing nucleic acid dye structures engineered for optimized fluorescent signatures with minimal spillover and spread.

Excitation: 636 nm
Emission: 756 nm
Laser: 633–640 nm (Red)

Target Information

CD16 encodes a receptor that binds the Fc portion of IgG, mediating immune complex clearance and cellular cytotoxicity.

• Gene: FCGR3A (chromosome 1)
• Expressed on NK cells (transmembrane form)
• FCGR3B expressed on neutrophils (PI linkage form)
  Mutations are associated with Immunodeficiency 20, Herpes Zoster, and susceptibility to lupus and viral infections.
  Alternative splicing produces multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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