Thermo Fisher Scientific SRC Monoclonal Antibody (5B8)
SRC 단백질을 인식하는 Mouse IgG1 단클론 항체로 Western blot과 ELISA에 사용 가능. A431 세포를 양성 대조군으로 권장. 세포 성장, 신호전달 및 암 관련 연구에 적합. -20°C에서 보관하며 동결/해동 반복은 피해야 함.
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Applications
Western Blot (WB)
- Tested Dilution: 4 µg/mL
ELISA
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B8 |
| Immunogen | Recombinant human protein purified from E.coli (His-src) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 4 mg/mL |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_1958783 |
Product Specific Information
A suggested positive control for this product is A431 cells.
Target Information
SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. It regulates growth and differentiation of eukaryotic cells by associating with the cytoplasmic region of various cell surface receptors to facilitate signal transduction. SRC also plays roles in cytoskeletal organization, cell cycle regulation, and apoptosis.
In humans, the SRC gene is located on chromosome 20. Its activity is regulated by phosphorylation at two tyrosine residues (Tyr416 and Tyr527), which have opposing effects: phosphorylation at Tyr416 activates the enzyme, while phosphorylation at Tyr527 inhibits it.
Mutations in SRC may contribute to malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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