Thermo Fisher Scientific CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), Super Bright 780, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.25 µg)/test
• View 17 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• eFluor 450
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

Description:
The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells.

Applications Reported:
This HIR2 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
This antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test).

Fluorochrome Characteristics:
Super Bright 780 is a tandem dye excited by the violet laser (405 nm) and emits at 780 nm. Recommended detection filter: 780/60 bandpass.

Usage Recommendations:

• When using multiple Super Bright dye-conjugated antibodies, use Super Bright Staining Buffer (Product # SB-4401-42) to minimize non-specific interactions.
• Compensation values may vary between beads and cells; it is recommended to set compensation using stained cells.
• Protect the dye and samples from light due to photo-induced oxidation sensitivity.
• Samples can be fixed with IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.

Excitation/Emission: 405 nm / 780 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD235a (Glycophorin A, GPA):
A single-pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a carries the M and N blood group antigens and provides a mucin-like barrier that reduces red cell aggregation. It also serves as a receptor for certain viruses and bacterial adhesins (e.g., Sandei virus, parvovirus, Streptococcus Hsa adhesin).

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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