Thermo Fisher Scientific Vimentin Monoclonal Antibody (V9), Alexa Fluor 488
Alexa Fluor 488로 표지된 Vimentin 단클론 항체(V9)로, 다양한 종의 중간 필라멘트 단백질 검출에 적합. Western blot, IHC, ICC/IF에 사용 가능하며 높은 특이성과 재현성을 제공. 단백질 G 정제, 액상 형태로 4°C 암소 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Horse, Cat, Gerbil, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | V9 |
| Immunogen | Purified vimentin from pig eye lens |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2662892 |
Available Formats
- Unconjugated (MA5-11883)
- Alexa Fluor 420 (758-9897-82)
- Alexa Fluor 555 (MA5-11883-A555)
- Alexa Fluor 647 (MA5-11883-A647)
- Alexa Fluor Plus 594 (755-9897-82)
- Alexa Fluor Plus 750 (757-9897-82)
- Custom conjugation available upon request
Product Specific Information
The MA5-11883-A488 immunogen is purified vimentin from pig eye lens.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular protein transport between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific IFPs. It plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to filament breakdown and cytoskeletal rearrangement, facilitating cholesterol transport to mitochondria and increasing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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