Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Alexa Fluor 660, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Alexa Fluor 660, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV / Violet / Yellow series
• eFluor 450 / 506
• FITC
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• Super Bright series
• NovaFluor Blue / Red / Violet / Yellow series
• Functional Grade
• Custom Conjugation Available

Product Specific Information

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta subunits, is essential for assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and mature T cells. Crosslinking via immobilized UCHT1 triggers intracellular signaling leading to activation and proliferation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test (defined as staining a sample in 100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test.

Alexa Fluor 660 emits at 690 nm and is suitable for spectral cytometers, multiplexed with APC, Alexa Fluor 647, and Alexa Fluor 700.
Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, encoded by genes on chromosome 11. These subunits contain cytoplasmic tails involved in signal transduction via tyrosine-based motifs. CD3 is expressed on 68–82% of peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells, but not on B or NK cells. CD3 expression abnormalities are linked to certain autoimmune diseases and immunodeficiencies.

For Research Use Only. Not for diagnostic use or resale without authorization.

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