Thermo Fisher Scientific Avidin Monoclonal Antibody (A3G7)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-Dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A3G7

Immunogen

Egg white avidin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

7 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_557671

Product Specific Information

MA1-21417 is specific for egg white avidin. No cross reaction is seen with human and animal serum proteins and streptavidin. The antibody reacts with avidin without interfering with its biotin-binding activity.

The predicted molecular weight of MA1-21417 is 17 kDa.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. This protein contains four identical subunits having a combined molecular mass of 67,000 to 68,000 daltons. Each subunit binds one molecule of biotin, and studies have shown that tryptophan and lysine are involved in the binding site for biotin. The sequencing of the subunit indicates it consists of 128 amino acids. Avidin has an isoelectric point of 10-10.5 and is very soluble in water and salt solutions. Avidin is stable over a wide range of pH values and temperatures. Extensive chemical modification has little effect on the activity of avidin, making it useful for detection and protein purification. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.