Thermo Fisher Scientific ERO1L Recombinant Rabbit Monoclonal Antibody (11H3L5)
ERO1L 단백질을 표적으로 하는 Thermo Fisher의 재조합 토끼 단클론 항체. Western blot 및 ICC/IF에 검증됨. 높은 특이성과 일관된 로트 품질 제공. 인간 및 생쥐 반응성, 단기 4°C 보관 및 장기 -20°C 보관.
- 카탈로그번호
- 702709
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific ERO1L Recombinant Rabbit Monoclonal Antibody (11H3L5)
Applications
Western Blot (WB)
- Tested Dilution: 2.5 µg/mL
- View 2 publications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 5 µg/mL
- References
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 11H3L5 |
| Immunogen | Protein corresponding to human ERO1L (aa22–aa468) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2716886 |
Product Specific Information
This antibody is predicted to react with Monkey, Pig, and Bovine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
ERO1L (endoplasmic oxidoreductin-1-like protein) is localized in the ER membrane as a peripheral membrane protein on the lumenal side. Its association with ERP44 is essential for retention in the ER and is unique in coupling oxygen reduction to de novo disulfide formation.
ERO1L is broadly distributed at low levels in several tissues, with highest levels in the upper digestive tract, and is stimulated by hypoxia via HIF signaling. It generally exists as a monomer but can function as a monomer or homodimer and interacts with PDILT.
After translation, ERO1L undergoes n-glycosylation, and its disulfide bonds form the redox-active center. The Cys-94/Cys-99 disulfide bond accepts electrons from P4HB and transfers them to the active site disulfide Cys-394/Cys-397. ERO1L oxidizes P4HB/PDI isomerase through direct disulfide exchange and associates with ERP44.
Biological roles:
- Immunoglobulin folding
- Oxidative stress response
- Release of unfolded cholera toxin during Vibrio cholerae infection
- ER stress-induced CHOP-dependent apoptosis via IP3R1 activation
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일: 702709_ERO1L_Q96HE7-1_Rabbit.svg, 702709_ERO1L_Q96HE7-1_Rabbit_PDP.jpeg)
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.