Thermo Fisher Scientific ERO1L Recombinant Rabbit Monoclonal Antibody (11H3L5)

Applications

Western Blot (WB)

• Tested Dilution: 2.5 µg/mL
• View 2 publications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 5 µg/mL
• References

Product Specifications

Product Specific Information

This antibody is predicted to react with Monkey, Pig, and Bovine.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

ERO1L (endoplasmic oxidoreductin-1-like protein) is localized in the ER membrane as a peripheral membrane protein on the lumenal side. Its association with ERP44 is essential for retention in the ER and is unique in coupling oxygen reduction to de novo disulfide formation.

ERO1L is broadly distributed at low levels in several tissues, with highest levels in the upper digestive tract, and is stimulated by hypoxia via HIF signaling. It generally exists as a monomer but can function as a monomer or homodimer and interacts with PDILT.

After translation, ERO1L undergoes n-glycosylation, and its disulfide bonds form the redox-active center. The Cys-94/Cys-99 disulfide bond accepts electrons from P4HB and transfers them to the active site disulfide Cys-394/Cys-397. ERO1L oxidizes P4HB/PDI isomerase through direct disulfide exchange and associates with ERP44.

Biological roles:

• Immunoglobulin folding
• Oxidative stress response
• Release of unfolded cholera toxin during Vibrio cholerae infection
• ER stress-induced CHOP-dependent apoptosis via IP3R1 activation

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일: 702709_ERO1L_Q96HE7-1_Rabbit.svg, 702709_ERO1L_Q96HE7-1_Rabbit_PDP.jpeg)