Thermo Fisher Scientific CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), Brilliant Violet 650, eBioscience

Thermo Fisher Scientific CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), Brilliant Violet 650, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 563
• Brilliant Violet 480
• Brilliant Violet 786
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

The eBio5E10 monoclonal antibody reacts with human CD90 (Thy-1), a 25–35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, and subsets of fetal liver, cord blood, and bone marrow cells. CD90 plays a role in T cell adhesion and signal transduction.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-diluted and tested on HEL cells at 5 µL (0.125 µg)/test.
A test is defined as the amount of antibody that stains a cell sample in 100 µL final volume (10⁵–10⁸ cells/test).

Brilliant Violet™ 650 (BV650):
Tandem dye emitting at 649 nm, for use on cytometers with a 405 nm violet laser. Ensure instrument compatibility.

Staining Recommendations:
When using multiple polymer dyes (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
BV650 is sensitive to photo-induced oxidation. Protect vial and samples from light.

Fixation:
Samples may be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark. BV650 is not compatible with methanol-based fixation.

Excitation / Emission / Laser:
407 nm / 649 nm / Violet Laser.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD90 (Thy-1) antigen is a GPI-linked glycoprotein of the Immunoglobulin superfamily. Expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts. Plays a role in cell-cell interactions during synaptogenesis and neural events.
Used as a marker for stem cells and axonal processes of mature neurons.
Associated diseases include nasopharyngeal carcinoma and thymoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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