Thermo Fisher Scientific P-Glycoprotein Monoclonal Antibody (C219)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:600

View 26 publications 26 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25

-

Immunohistochemistry (Frozen) (IHC (F))

5-10 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 4 publications 4 publications

Flow Cytometry (Flow)

5-10 µg for 1x10^6 cells

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Fish, Hamster, Human, Mouse, Rat, Zebrafish

Published species

Dog, Fish, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C219

Immunogen

SDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:P-Glycoprotein (ABCB1),uniProtId:P08183-1,ncbiNodeId:9606,antigenRange:1-1280,antigenLength:1280,antigenImageFileName:MA1-26528_P-Glycoprotein_ABCB1_P08183-1_House_mouse.svg,antigenImageFileNamePDP:MA1-26528_P-Glycoprotein_ABCB1_P08183-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:P-Glycoprotein (ABCB1),uniProtId:P21448-1,ncbiNodeId:10026,antigenRange:1-1276,antigenLength:1276,antigenImageFileName:MA1-26528_P-Glycoprotein_ABCB1_P21448-1_House_mouse.svg,antigenImageFileNamePDP:MA1-26528_P-Glycoprotein_ABCB1_P21448-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.72 mg/mL

Purification

purified

Storage buffer

PBS with 1% BSA

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_795165

Product Specific Information

Recommended positive controls: Cell lines or frozen tissue, such as liver or colon.

MA1-26528 is expected to cross react with a wide range of mammals due to sequence homology.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

This clone recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, MDR1 P-glycoprotein (170kDa) and MDR3 P-glycoprotein (140kDa). In Western blot, boiling samples may reduce visibility of the protein.

For flow cytometry, cells should be fixed in 3.7% formaldehyde for 10 min at room temperature to permeabilize the cell membrane. Incubate cells with 5-10 µg antibody for 30-60 min at 4ºC.

Target Information

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.