Thermo Fisher Scientific P-Glycoprotein Monoclonal Antibody (C219)
P-Glycoprotein 단백질을 인식하는 C219 클론 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. 인간 및 다종 포유류 반응성. 비결합형 액상 항체로, PBS/1% BSA 용액에 보관. 약물 내성 연구 및 ABC 수송체 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution / Concentration | Publications |
|---|---|---|
| Western Blot (WB) | 1:50–1:600 | 26 |
| Immunohistochemistry (IHC) | – | 5 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–10 µg/mL | – |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 4 |
| Flow Cytometry (Flow) | 5–10 µg for 1×10⁶ cells | – |
| ELISA | Assay-dependent | – |
| Immunoprecipitation (IP) | Assay-dependent | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Fish, Hamster, Human, Mouse, Rat, Zebrafish |
| Published Species | Dog, Fish, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C219 |
| Immunogen | SDS-solubilized plasma membranes of multidrug resistant CHO and human cell lines |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.72 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C (short term); -20°C (long term). Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_795165 |
Product Specific Information
- Recommended positive controls: Cell lines or frozen tissue (e.g., liver or colon)
- Expected to cross-react with multiple mammalian species due to sequence homology
- Store as a concentrated solution; centrifuge briefly before opening
- Recognizes conserved amino acid sequences (VQEALD, VQAALD) in MDR1 (~170 kDa) and MDR3 (~140 kDa) P-glycoproteins
- Boiling samples may reduce WB signal intensity
- For flow cytometry: Fix cells with 3.7% formaldehyde for 10 min at room temperature, then incubate with 5–10 µg antibody for 30–60 min at 4°C
Target Information
P-Glycoprotein is a member of the ATP-binding cassette (ABC) transporter superfamily. These proteins transport diverse molecules across cellular membranes and are grouped into seven subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).
This protein belongs to the MDR/TAP subfamily, associated with multidrug resistance. It functions as an ATP-dependent efflux pump for xenobiotic compounds, reducing intracellular drug accumulation and mediating resistance to anticancer agents.
It also plays a role as a transporter in the blood-brain barrier.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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