Thermo Fisher Scientific APE1 Polyclonal Antibody
APE1 단백질을 인식하는 Rabbit Polyclonal Antibody로, Human, Mouse, Rat에 반응합니다. Western blot, IHC, ICC, IP, ChIP 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS 기반 버퍼에 보관되며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
| ChIP assay (ChIP) | 1.5 µg/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–318 of Human APE1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.34 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546633 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, PC-12
- Predicted reactivity: Mouse (94%), Rat (93%), Dog (95%), Pig (94%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (93%)
- Store product as a concentrated solution. Centrifuge briefly before opening the vial.
Target Information
Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional enzyme involved in DNA repair and redox regulation of transcription factors such as Fos, Jun, NF-κB, Myb, HIF-1α, CREB, and Pax. It participates in cell cycle control (p53) and apoptosis. APE repairs abasic sites caused by oxidative stress, radiation, or chemical damage. Expression levels vary by tissue and developmental stage, and regulation occurs partly via phosphorylation by casein kinase II. APE levels increase upon reoxygenation of hypoxic cells and in some malignant tissues, while decreases are associated with apoptosis induction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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