Thermo Fisher Scientific Ephrin B1, B2, B3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:5,000

-

ELISA (ELISA)

1:10,000-1:50,000

-

Product Specifications

Species Reactivity

Human, Mouse, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Ephrin B1-B3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-terminal portion of human Ephrin B1, B2, B3 conjugated to Keyhole Limpet Hemocyanin (KLH). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ephrin B1/B2/B3,uniProtId:P52799-1,ncbiNodeId:9606,antigenRange:333,antigenLength:333,antigenImageFileName:600-401-MP0_Ephrin_B1B2B3_P52799-1_Rabbit.svg,antigenImageFileNamePDP:600-401-MP0_Ephrin_B1B2B3_P52799-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:Ephrin B1/B2/B3,uniProtId:P98172-1,ncbiNodeId:9606,antigenRange:346,antigenLength:346,antigenImageFileName:600-401-MP0_Ephrin_B1B2B3_P98172-1_Rabbit.svg,antigenImageFileNamePDP:600-401-MP0_Ephrin_B1B2B3_P98172-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:Ephrin B1/B2/B3,uniProtId:Q15768-1,ncbiNodeId:9606,antigenRange:340,antigenLength:340,antigenImageFileName:600-401-MP0_Ephrin_B1B2B3_Q15768-1_Rabbit.svg,antigenImageFileNamePDP:600-401-MP0_Ephrin_B1B2B3_Q15768-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.05 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human Ephrin B1, B2, B3. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with Ephrin-B1 to human, mouse, rat, and Gallus gallus; 100% homology with Ephrin-B2 to mouse; 100% homology with Ephrin-B3 to human and mouse.

Target Information

Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.