Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Brilliant Violet 605, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 661, 737
• Brilliant Violet 480
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series (multiple wavelengths)
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds to MHC class II molecules and plays a key role in T-cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

• Applications Reported: Flow cytometric analysis.
• Applications Tested: Validated using mouse splenocytes (≤0.5 µg/test). Optimize titration for best assay performance.
• Fluorochrome: Brilliant Violet™ 605 (BV605), emits at 605 nm, suitable for violet (405 nm) laser.

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity:
Sensitive to photo-induced oxidation. Protect from light.

Fixation Compatibility:
Samples can be fixed with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark. Not compatible with methanol-based fixation.

Laser Parameters:
Excitation: 407 nm
Emission: 605 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

CD4 antigen is involved in recognition of MHC class II molecules and serves as a co-receptor for HIV. Expressed mainly in T-helper lymphocytes, and also in monocytes, macrophages, dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and specific regions of brain and gut. CD4 mediates T-cell activation and may contribute to neuronal damage in immune-mediated CNS diseases. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지