
Thermo Fisher Scientific pcDNA3.1 Directional TOPO Expression Kit, 20 Reactions
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Invitrogen™
pcDNA™3.1 Directional TOPO™ Expression Kit
The pcDNA™3.1 Directional TOPO™ Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO™ for five-minute directional cloning and subsequent high-level expression.Directional Cloning자세히 알아보기
The pcDNA™3.1 Directional TOPO™ Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO™ for five-minute directional cloning and subsequent high-level expression.
Directional Cloning technology facilitates expression experiments because:
• A proofreading enzyme is used for fewer errors in cloned genes
• Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation
In addition, pcDNA3.1D/V5-His-TOPO™ provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody and rapid purification on nickel-chelating resin.
사양
구성 또는 유도성 시스템Constitutive
배달 유형Transfection
프로모터CMV
제품 유형TOPO Expression Kit
선택 제제(진핵)Geneticin™ (G-418)
단백질 태그His Tag (6x), V5 Epitope Tag
클로닝 방법Directional TOPO
수량20 Reactions
벡터Directional TOPO Vectors, pcDNA
제품라인TOPO™, pcDNA™
용도(애플리케이션)Constitutive Expression
Unit SizeEach
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