Invitrogen™

BLOCK-iT™ Adenoviral RNAi Expression System

The pAd/BLOCK-iT™-DEST RNAi Gateway™ Vector can be used for the efficient delivery and transient expression of a short hairpin RNA자세히 알아보기

The pAd/BLOCK-iT™-DEST RNAi Gateway™ Vector can be used for the efficient delivery and transient expression of a short hairpin RNA (shRNA) in vivo from an adenoviral vector. A novel cloning process places an ∼50-bp DNA oligonucleotide immediately following a U6 pol III promoter into the BLOCK-iT™ U6 entry vector. After efficient recombination of the entry vector into the pAd/BLOCK-iT™-DEST vector, followed by viral production and transduction, the shRNA driven by the U6 promoter can be transiently expressed in most dividing or non-dividing mammalian cell types. The shRNA generated avoids the hosts defense mechanism and will be effective at producing the RNAi gene knockdown response.

The pAd/BLOCK-iT™-DEST vector (Figure 1) offers:

• A promoterless region surrounded by _att_R sites for efficient recombination with the _att_L-flanked U6 Gateway™ entry vector containing the RNAi cassette or any _att_L-flanked promoter and gene sequence
• All of the required components for efficient adenoviral packaging and delivery of the shRNA of interest. With this vector, transient analysis of gene knockdown in both dividing and non-dividing mammalian cell types and animal models can be achieved.

사양

제품 유형RNAi Expression Vector Kit

클로닝 방법Gateway™

구성 또는 유도성 시스템Constitutive

배달 유형Adenoviral

RNAi 유형shRNA

벡터pAd

프로모터U6

제품라인BLOCK-iT™

수량20 reactions

Unit SizeEach