Thermo Fisher Scientific E-cadherin Recombinant Rabbit Monoclonal Antibody (SY0287)
E-cadherin 단백질을 인식하는 recombinant rabbit monoclonal antibody로, WB, IHC, ICC, Flow 분석에 적합합니다. 인간 시료에 반응하며 높은 특이성과 재현성을 제공합니다. Protein A 정제, 액상 형태로 안정적이며 연구용으로 사용됩니다.
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Thermo Fisher Scientific E-cadherin Recombinant Rabbit Monoclonal Antibody (SY0287)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY0287 |
| Immunogen | Synthetic peptide within human E-Cadherin aa 580–630 (Extracellular) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2898875 |
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the calcium-dependent adhesion protein superfamily. It encodes a glycoprotein responsible for cell-cell adhesion, consisting of five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. Mutations in this gene are associated with various cancers including gastric, breast, colorectal, thyroid, and ovarian cancer. Loss of E-cadherin function contributes to cancer progression by increasing proliferation, invasion, and metastasis. The ectodomain mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. E-cadherin, encoded by the CDH1 gene on chromosome 16, plays a crucial role in maintaining tissue architecture and integrity.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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