Thermo Fisher Scientific CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), PE-Cyanine7, eBioscience
인간 CD90(Thy-1) 인식 단클론 항체로, PE-Cyanine7 형광 표지된 Flow Cytometry용 시약입니다. 인간 세포에서 CD90 발현 분석에 적합하며, 5 µL/test로 사용됩니다. 광민감성이 있으므로 어둡게 보관하며 4°C에서 냉장 보관합니다.
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Applications
Tested Application
- Flow Cytometry (Flow)
Tested Dilution
- 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio5E10 (5E10) |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573375 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 563
- Brilliant Violet 480
- Brilliant Violet 650
- Brilliant Violet 786
- FITC
- PE
- PE-Cyanine5
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 436
- Request custom conjugation
Product Specific Information
The eBio5E10 monoclonal antibody reacts with human CD90 (Thy-1), a 25–35 kDa receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, and subsets of fetal liver, cord blood, and bone marrow cells.
CD90 is involved in T-cell adhesion and signal transduction.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested using human erythroleukemia (HEL) cells. Recommended usage is 5 µL (0.25 µg) per test, defined as the amount sufficient to stain a sample in 100 µL final volume. Cell numbers may range from 10⁵ to 10⁸ cells/test.
Light Sensitivity: This tandem dye is sensitive to photo-induced oxidation; protect from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
Excitation/Emission: 488–561 nm / 775 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD90 (Thy-1) is a GPI-linked glycoprotein of the Immunoglobulin superfamily. It is expressed in murine T cells, thymocytes, neural cells, Kupffer’s cells, and fibroblasts. CD90 plays roles in cell-cell and cell-ligand interactions, especially during synaptogenesis in the brain. It serves as a marker for various stem cells and neuronal axons. Structural studies of CD90 contributed to the identification of the Immunoglobulin superfamily and the first description of a vertebrate GPI anchor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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