Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), APC, eBioscience

Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), APC, eBioscience™

Applications and Tested Dilutions

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• Alexa Fluor 700
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1 (Arg1), a cytosolic enzyme.
This clone is compatible with standard intracellular staining protocols and the Foxp3/Transcription Factor Staining Buffer Set.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00) and standard protocols.
Refer to BestProtocols®: Protocol A for cytoplasmic intracellular proteins.
Recommended use ≤ 1.0 µg/test (100 µL final volume). Adjust cell number empirically (10⁵–10⁸ cells/test). Titrate for optimal performance.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme that converts L-arginine to urea and L-ornithine, completing the urea cycle.
Polyamines produced are essential for cell proliferation and detoxification.
Arginase 1 down-regulates nitric oxide production by depleting substrate for NO synthase.
Expressed in liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells in both human and mouse.
In humans, expression occurs in blood neutrophils but not CCR3+ granulocytes.
In mice, Arginase 1 marks alternatively activated macrophages (M2a).
Associated with myeloid cells infiltrating tumors and hepatocellular carcinomas.
Defects cause argininemia, an autosomal recessive disorder with hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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