Thermo Fisher Scientific XPF Monoclonal Antibody (OTI2A1), TrueMAB
XPF 단백질을 표적으로 하는 Mouse monoclonal antibody (Clone OTI2A1). Western blot, ICC/IF, Flow Cytometry에 적합. Human, Mouse, Rat, Dog 반응성. Lyophilized 형태로 제공되며, PBS buffer(8% trehalose)로 안정화됨. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A1 |
| Immunogen | Full length human recombinant protein of human ERCC4 produced in HEK293 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The ERCC1/XPF endonuclease complex is involved in DNA repair mechanisms, including:
- Nucleotide excision repair (NER) for UV-induced lesions and bulky chemical adducts
- Recombination repair of interstrand cross-links
In mammalian cells:
- XPG cleaves the 3′ side of the DNA lesion
- ERCC1/XPF complex makes the 5′ incision
This complex likely also processes partially repaired recombination intermediates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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