Thermo Fisher Scientific PIK3C2A Monoclonal Antibody (OTI3C10), TrueMAB

Applications

• Western Blot (WB): 1:2,000 dilution tested

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Class II PI3 kinases, including PIK3C2A, phosphorylate the 3 position of PI or PI4P. Although less studied than Class I PI3 kinases, Class II enzymes are involved in receptor-mediated cellular processes such as insulin signaling, neuronal survival, and growth factor signaling.
PI3-kinases contribute to pathways regulating cell proliferation, oncogenic transformation, cell survival, migration, and intracellular trafficking.
PIK3C2A contains a lipid kinase catalytic domain and a C-terminal C2 domain, which acts as a calcium-dependent phospholipid-binding motif mediating membrane translocation and protein-protein interactions.
Its PI3-kinase activity is not sensitive to nanomolar levels of wortmanin and can be activated by insulin, potentially participating in integrin-dependent signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.