Thermo Fisher Scientific PIK3C2A Monoclonal Antibody (OTI1B5), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7 K MWCO, 0.5 mL, Product #89882).

Target Information

The Class II PI3 kinases, including PIK3C2A, phosphorylate the 3-position of PI or PI4P. Although less studied than Class I PI3 kinases, Class II enzymes are implicated in receptor-mediated processes such as insulin signaling, neuronal survival, and growth factor signaling.
PI3-kinases are involved in pathways regulating cell proliferation, oncogenic transformation, survival, migration, and intracellular trafficking.
PIK3C2A contains a lipid kinase catalytic domain and a C-terminal C2 domain, characteristic of Class II PI3-kinases. C2 domains act as calcium-dependent phospholipid-binding motifs mediating membrane translocation and protein-protein interactions.
The PI3-kinase activity of PIK3C2A is not sensitive to nanomolar levels of wortmannin and can be activated by insulin, suggesting a role in integrin-dependent signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.