Thermo Fisher Scientific MED4 Recombinant Rabbit Monoclonal Antibody (8T5C9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

8T5C9

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 150-250 of human MED4 (Q9NPJ6). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MED4,uniProtId:Q9NPJ6-1,ncbiNodeId:9606,antigenRange:150-250,antigenLength:270,antigenImageFileName:MA5-42613_MED4_Q9NPJ6-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42613_MED4_Q9NPJ6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.8 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911754

Product Specific Information

Positive test controls include: TE-1, Jurkat, U-87MG, Mouse lung, Rat liver. The target is usually found in the following locations: Nucleus.

Immunogen sequence: KYAHRISASN AVCAPLTWVP GDPRRPYPTD LEMRSGLLGQ MNNPSTNGVN GHLPGDALAA GRLPDVLAPQ YPWQSNDMSM NMLPPNHSSD FLLEPPGHNK E

Target Information

The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for activation of polymerase II transcribed genes. MED4 appears to be a core Mediator subunit and is found in nearly all Mediator preparations (Sato et al., 2004).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.