Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Brilliant Ultra Violet 496, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 615, 737, 805
• Brilliant Violet 421, 605, 650, 711
• eFluor 450, 506
• FITC
• NovaFluor Blue 610-70S, 660-120S
• NovaFluor Red 710, 725
• NovaFluor Yellow 590, 660
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed on developing and mature B cells but not plasma cells. It may play a role in B-cell activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested for flow cytometry using human peripheral blood cells. Recommended 5 µL (1.0 µg) per test in 100 µL final volume.
• Cell Range: 10⁵–10⁸ cells/test (empirically determined)

Brilliant Ultra Violet™ 496 (BUV496):
Tandem dye emitting at 496 nm; requires cytometer equipped with a 355 nm UV laser.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal effect on brightness.

Excitation/Emission:
Excitation: 350 nm; Emission: 496 nm; Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and most malignant B cells, some T lymphocytes, and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 remains at the cell surface after antibody binding, making it a valuable therapeutic target for B-cell malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지