
Thermo Fisher Scientific EphA3 (extracellular) Polyclonal Antibody
EphA3 단백질의 세포외 영역을 인식하는 Rabbit Polyclonal Antibody. Western blot, IHC(F), Flow Cytometry에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 인간, 생쥐, 랫트 반응성.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:200 |
| Flow Cytometry (Flow) | 5 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)RARGTNVTISSLK, corresponding to amino acid residues 488–500 of human EphA3 (Extracellular, N-terminus) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution:
Add 25 µL, 50 µL, or 0.2 mL of double distilled water (DDW) depending on the sample size.
The antibody ships as a lyophilized powder at room temperature. Upon arrival, store at -20°C.
The reconstituted solution can be stored at 4°C, protected from light, for up to 1 week.
For longer storage, aliquot and keep at -20°C. Avoid repeated freeze/thaw cycles.
Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
EPHA3 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family.
EPH and EPH-related receptors play roles in developmental processes, particularly in the nervous system.
EPHA3 encodes a protein that binds ephrin-A ligands.
Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a cysteine-rich domain and two fibronectin type III repeats.
Ephrin receptors are divided into two groups based on extracellular domain sequence similarity and ligand binding affinities.
EPHA3 binds ephrin-A ligands.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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