Thermo Fisher Scientific H3 alpha Polyclonal Antibody
다양한 종(Rat, Human, Mouse, Sheep)에 반응하는 H3 alpha 폴리클로날 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. Protein A로 정제된 고순도 항체로, 안정적인 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat, Human, Mouse, Sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human HIF3 alpha, amino acids 131–230 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Cell growth and viability are compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1 alpha, HIF-1 beta (also designated Arnt 1), EPAS-1 (also designated HIF-2 alpha), and HIF-3 alpha, induce glycolysis, erythropoiesis, and angiogenesis to restore oxygen homeostasis. These factors belong to the Per-Arnt-Sim (PAS) domain transcription factor family. Under hypoxic conditions, HIF-1 alpha is upregulated and forms a heterodimer with Arnt 1 to create the HIF-1 complex, which binds to hypoxia-responsive elements (HRE) in target genes to activate transcription.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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