Thermo Fisher Scientific Syk Polyclonal Antibody, FITC
Syk 단백질을 인식하는 FITC 결합 폴리클로날 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 사용 가능하며, 형광 분석에 적합합니다. 액상 형태로 제공되며, -20°C 어두운 곳에 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 100–150 on human Syk protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, store in the dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases that serve as potential drug targets for allergic disorders and autoimmune diseases. SYK may also play a role in cancer. Upon activation of B cell antigen receptor (BCR) or high-affinity IgE receptor (FcεRI), Syk translocates to the plasma membrane and phosphorylates downstream adaptor proteins, initiating signaling pathways involved in calcium mobilization, cytoskeleton remodeling, and gene transcription.
Syk interacts with receptor assemblies through its SH2 domains binding to ITAM motifs phosphorylated by Src-family kinases, which also activate Syk by phosphorylation of its activation loop.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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