Thermo Fisher Scientific PRDX5 Recombinant Rabbit Monoclonal Antibody (002)
인체 PRDX5 단백질을 인식하는 Thermo Fisher의 재조합 토끼 단클론 항체입니다. Western blot, ICC/IF, IP 등 다양한 응용에 적합하며, 높은 특이성과 일관된 품질을 제공합니다. 방부제 무첨가 포뮬레이션으로 장기 보관 시 안정적입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:20–1:100 |
| Immunoprecipitation (IP) | 4–8 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 002 |
| Immunogen | Recombinant Human Peroxiredoxin 5/PRDX5 protein (Met53–Leu214) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.24 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785353 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to avoid contamination.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal production clones.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to large rabbit immune repertoire
This antibody has specificity for Human Peroxiredoxin 5 (PRDX5).
Target Information
Peroxiredoxin (Prx) is a peroxidase family associated with cell proliferation, differentiation, and apoptosis. Mammalian Prx proteins (approximately 50 isoforms) contain active site cysteine residues and exhibit thiol-specific antioxidant activity.
Mammalian Prx members are categorized into:
- Typical 2-Cys Prx
- Atypical 2-Cys Prx
- 1-Cys Prx
Except for Prx VI (1-Cys subgroup), other 2-Cys Prx isotypes possess thioredoxin-dependent peroxidase (TPx) activity using thioredoxin, thioredoxin reductase, and NADPH as reducing systems.
Mammalian Prxs (20–30 kDa) vary in subcellular localization:
- Prx I, II, VI: cytosol
- Prx III: mitochondria
- Prx IV: ER and secretion
- Prx V: peroxisome, mitochondria, and cytosol
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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