Thermo Fisher Scientific HPV Type 16 E6 Recombinant Mouse Monoclonal Antibody (Nb9)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Neutralization (Neu)

Assay-dependent

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Mouse / VHH-mFc

Expression System

CHO cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

Nb9

Immunogen

Recombinant HPV 16 E6/Protein E6.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Compatible with Direct ELISA

Target Information

Plays a major role in the induction and maintenance of cellular transformation. Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins. E6 associates with host E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation. In turn, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development. The complex E6/E6P targets several other substrates to degradation via the proteasome including host NFX1-91, a repressor of human telomerase reverse transcriptase (hTERT). The resulting increased expression of hTERT prevents the shortening of telomere length leading to cell immortalization. Other cellular targets including Bak, Fas-associated death domain-containing protein (FADD) and procaspase 8, are degraded by E6/E6AP causing inhibition of apoptosis. E6 also inhibits immune response by interacting with host IRF3 and TYK2. These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.