Invitrogen™

Champion™ pET302/NT-His and pET303/CT-His Vector Kit

The Champion™ pET302/NT-His and pET303/CT-His Vector Kit is designed for cloning a gene of interest via restriction enzyme(s) and ligase자세히 알아보기

The Champion™ pET302/NT-His and pET303/CT-His Vector Kit is designed for cloning a gene of interest via restriction enzyme(s) and ligase (REaL) and subsequent high-level expression from the strong bacteriophage T7 promoter. In addition to the T7 promoter, each vector contains only the necessary functional elements and an N- or C-terminal 6xHis tag (pET302/NT-His and pET303/CT-His, respectively) for convenient purification and detection (Figure 1). Expression levels obtained from these vectors may be higher than those obtained from another suppliers pET vector (Figure 2). To maximize expression, use with MagicMedia™ E. coli Expression Medium.

Contents and Storage:

The Champion™ pET302/NT-His and pET303/CT-His Vector Kit includes 6 μg each of pET302/NT-His and pET303/CT-His and 10 μg of a control vector. Store at -20“C. All components are guaranteed stable for 6 months when properly stored.

사양

구성 또는 유도성 시스템Inducible

유도제IPTG

프로모터T7

제품 유형DNA Cloning Vector Kit

선택 제제(진핵)None

항생제 내성 박테리아Ampicillin (AmpR)

단백질 태그His Tag (6x)

클로닝 방법Restriction Enzyme/MCS

수량10 μg

벡터pET, pDEST

제품라인Champion™

Unit SizeEach