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Thermo Fisher Scientific pENTR 5`-TOPO TA Cloning Kit, with One Shot TOP10 Chemically Competent E. coli

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Invitrogen pENTR 5'-TOPO TA Cloning Kit은 TOPO 클로닝과 MultiSite Gateway 기술을 결합해 5분 내 PCR 산물 클로닝을 구현합니다. 다양한 프로모터 선택 가능하며, 고효율(≥85%) 클로닝을 제공합니다. One Shot TOP10 Competent E. coli 포함.

카탈로그번호: K59120
브랜드: Thermo Fisher Scientific
카테고리: Other Organics
판매단위: pk

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Thermo Fisher Scientific K59120 pENTR 5`-TOPO TA Cloning Kit, with One Shot TOP10 Chemically Competent E. coli Each pk판매 단위 pk ·

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Thermo Fisher Scientific · Thermo Fisher Scientific pENTR 5`-TOPO TA Cloning Kit, with One Shot TOP10 Chemically Competent E. coli

Invitrogen™ pENTR™ 5'-TOPO™ TA Cloning™ Kit (with One Shot™ TOP10 Chemically Competent E. coli)

The pENTR™ 5'-TOPO™ TA Cloning™ Kit combines Invitrogen's TOPO™ Cloning and MultiSite Gateway™ Technologies to facilitate five-minute, one-step cloning of a Taq polymerase-amplified PCR product encoding a eukaryotic promoter of interest into a MultiSite Gateway™ entry vector with ≥85% efficiency.

Once cloned, your eukaryotic promoter may be transferred from the pENTR™ 5'-TOPO™ vector to a suitable MultiSite Gateway™ destination vector in a MultiSite Gateway™ LR recombination reaction with other suitable entry clones to generate an expression construct.

This kit can be used in combination with:

ViraPower™ HiPerform Promoterless Lentiviral Gateway™ Vector Kit
MultiSite Gateway™ Three-Fragment Vector Construction Kit

Advantages

Choose the best promoter for your experiments and cell line by efficiently expressing your gene of interest from multiple promoter constructs
When used with ViraPower™ HiPerform™ Promoterless Lentiviral vector, efficiently transduce and express your gene of interest driven by the promoter of choice in both dividing and non-dividing mammalian cells

Key Features

Rapid PCR cloning with TOPO™ cloning technology for cloning the 5' element
attL4 and attR1 sites for compatibility with MultiSite Gateway™ LR reaction for increased flexibility

Kit Includes

pENTR™ 5'-TOPO™ TA Cloning™ Kit
One Shot™ TOP10 Chemically Competent E. coli (C4040-03)

ViraPower™ HiPerform™ Promoterless Lentiviral Gateway™ Expression System (A111451)
ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit (A11146)
MultiSite Gateway™ Three-Fragment Vector Construction Kit (12537-023)

For research use only. Not intended for any therapeutic or diagnostic use.