Thermo Fisher Scientific pENTR 5`-TOPO TA Cloning Kit, with One Shot TOP10 Chemically Competent E. coli

Invitrogen™ pENTR™ 5'-TOPO™ TA Cloning™ Kit (with One Shot™ TOP10 Chemically Competent E. coli)

The pENTR™ 5'-TOPO™ TA Cloning™ Kit combines Invitrogen's TOPO™ Cloning and MultiSite Gateway™ Technologies to facilitate five-minute, one-step cloning of a Taq polymerase-amplified PCR product encoding a eukaryotic promoter of interest into a MultiSite Gateway™ entry vector with ≥85% efficiency.

Once cloned, your eukaryotic promoter may be transferred from the pENTR™ 5'-TOPO™ vector to a suitable MultiSite Gateway™ destination vector in a MultiSite Gateway™ LR recombination reaction with other suitable entry clones to generate an expression construct.

This kit can be used in combination with:

• ViraPower™ HiPerform Promoterless Lentiviral Gateway™ Vector Kit
• MultiSite Gateway™ Three-Fragment Vector Construction Kit

Advantages

• Choose the best promoter for your experiments and cell line by efficiently expressing your gene of interest from multiple promoter constructs
• When used with ViraPower™ HiPerform™ Promoterless Lentiviral vector, efficiently transduce and express your gene of interest driven by the promoter of choice in both dividing and non-dividing mammalian cells

Key Features

• Rapid PCR cloning with TOPO™ cloning technology for cloning the 5' element
• attL4 and attR1 sites for compatibility with MultiSite Gateway™ LR reaction for increased flexibility

Kit Includes

• pENTR™ 5'-TOPO™ TA Cloning™ Kit
• One Shot™ TOP10 Chemically Competent E. coli (C4040-03)

Related SKUs

• ViraPower™ HiPerform™ Promoterless Lentiviral Gateway™ Expression System (A111451)
• ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit (A11146)
• MultiSite Gateway™ Three-Fragment Vector Construction Kit (12537-023)

For research use only. Not intended for any therapeutic or diagnostic use.

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