Thermo Fisher Scientific Tartrate Resistant Acid Phosphatase Polyclonal Antibody
Tartrate Resistant Acid Phosphatase(TRAP)에 특이적인 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. Human 및 Rat 반응성, Affinity chromatography로 정제. PBS/glycerol buffer로 안정적 보관 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 | – |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 1 publication |
| ELISA | 1 µg/mL | – |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 76–325 of human ACP5 (NP_0016021) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.99 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901600 |
Product Specific Information
- Positive Samples: BT-474, Jurkat
- Immunogen Sequence:
TGVQDINDKR FQETFEDVFS DRSLRKVPWY VLAGNHDHLG NVSAQIAYSK ISKRWNFPSP FYRLHFKIPQ TNVSVAIFML DTVTLCGNSD DFLSQQPERP RDVKLARTQL SWLKKQLAAA REDYVLVAGH YPVWSIAEHG PTHCLVKQLR PLLATYGVTA YLCGHDHNLQ YLQDENGVGY VLSGAGNFMD PSKRHQRKVP NGYLRFHYGT EDSLGGFAYV EISSKEMTVT YIEASGKSLF KTRLPRRARP
Target Information
Tartrate-resistant acid phosphatase (TRAP) is a basic iron-binding protein with high activity towards phosphoproteins, ATP, and 4-nitrophenyl phosphatase. It is detected in human alveolar macrophages, osteoclasts, spleen, and liver. Expression is increased in:
- Spleen and monocytes of patients with Gaucher’s disease
- Spleenocytes and circulating leukocytes of patients with hairy cell leukemia
- Spleen of patients with Hodgkin’s lymphoma
- Sera of patients undergoing active bone turnover
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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