Thermo Fisher Scientific IFIT3 Monoclonal Antibody (OTI1G1), TrueMAB
Human IFIT3 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, ICC/IF, Flow cytometry에 적합함. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 항바이러스 반응 및 세포 신호 연구용으로 최적화된 TrueMAB 제품.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G1 |
| Immunogen | Full length human recombinant protein of human IFIT3 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
IFIT3 is an IFN-induced antiviral protein that inhibits cellular and viral processes such as migration, proliferation, signaling, and replication. It enhances MAVS-mediated antiviral responses by bridging TBK1 to MAVS, leading to TBK1 activation and IRF3 phosphorylation. IFIT3 also exhibits antiproliferative activity by upregulating CDKN1A/p21 and CDKN1B/p27 through sequestration of COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 levels and downregulating MYC. It can negatively regulate the apoptotic effects of IFIT2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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