Thermo Scientific™

Pierce™ Firefly Luciferase Flash Assay Kit

The Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell자세히 알아보기

The Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

Features of the Firefly Luciferase Flash Assay Kit:

• Highly sensitive detection of firefly luciferase activity
• Use with Pierce Firefly Luciferase Signal Enhancer for best results (maximum signal)
• Working solution stable for up to 2 months at -20°C
• Assay reagents compatible with other firefly luciferases
• Amenable to automation
• Contains a universal cell lysis buffer and optimized flash assay reagent
• Safe non-radioactive assay

Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit contains reagents to measure the activity of firefly luciferase in mammalian whole cell lysates. Containing a convenient lysis buffer, assay buffer and firefly substrate, this kit provides a complete reagent system for the intracellular detection of promoter or pathway activity. Light output resulting from the luciferase reaction is captured using a luminometer and can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression. These reagents are optimized to work with Thermo Scientific Firefly Luc Plasmids; however, the Flash Assay Kit may be used to detect the activity of other firefly or ATP-dependent luciferases utilizing D-luciferin as a substrate.

Includes:
Cell lysis buffer, reaction buffer and substrate; purchase Luciferase Signal Enhancer separately.

Requires:
• Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
• For best results (maximum signal), use with Pierce Firefly Luciferase Signal Enhancer

Applications:
• Promoter studies for analyzing _cis_- regulatory elements and _trans_-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

Firefly luciferase is a 60kDa protein produced in nature by several species of the Lampyridae family of beetles which includes the genera Photinus and Luciola. Bioluminescent signal from firefly luciferase originates from the oxidation of D-luciferin. Light output captured using a luminometer can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression.

More Product Data
• Monitoring neuronal differentiation using multiplexed luciferase reporters
• Activation of the antioxidant response pathway by pesticide chemicals
• Versatile luciferases: microplate luminometers and flash luciferase assays

사양

유형Assay Kit

타겟Luciferase, Firefly Luciferase

수량100 Reaction Kit

어세이Reporter Enzyme, Luciferase Reporter Assay

호환 셀Mammalian Cells

검출 방법Bioluminescence

용도(장비)Luminometer (Microplate)

형식384-well plate, 96-well plate

라벨 유형Enzyme Labeled

기질 특성Chemical Substrate

기술Enhanced Chemiluminescence

기질 유형Luciferase Substrate

제품라인Pierce™

기질D-Luciferin

Unit SizeEach