Thermo Fisher Scientific Pierce Gaussia Luciferase Glow Assay Kit, 100 Reactions

Thermo Scientific™ Pierce™ Gaussia Luciferase Glow Assay Kit

The Thermo Scientific Pierce Gaussia Luciferase Glow Assay Kit provides a sensitive bioluminescence system to determine expression of a gene of interest with a luciferase reporter.
The extremely bright and stable bioluminescence signal (half-life greater than 1 hour) is produced in the presence of the Gaussia-Dura luciferase reporter due to oxidation of the luciferase substrate coelenterazine.

Gaussia luciferase is a 20 kDa protein from the marine copepod Gaussia princeps, offering greater protein stability and brightness than firefly or native Renilla luciferase. The enzyme is highly secreted into cell culture media, enabling live-cell monitoring of reporter activity. Light output correlates with the amount of Gaussia luciferase protein produced and can be used to determine promoter activity driving Gaussia expression.

Features

• Sensitive: highly sensitive detection of Gaussia luciferase activity
• Stable: increased signal stability
• Simple: does not require luminometer injectors
• Compatible: reagents compatible with other Gaussia luciferases
• Automation-friendly: suitable for high throughput assays
• Convenient: includes universal cell lysis buffer and optimized glow assay reagent
• Safe: enables non-radioactive assays

When used with Thermo Scientific Gaussia-Dura Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for detection of secreted or intracellular luciferase activity from promoter or pathway activation with extended signal stability.
The light output (emission maximum of 485 nm) indicates transcription of Gaussia luciferase and is proportional to promoter activity. This reaction does not require ATP or other cofactors.

The kit includes cell lysis buffer, reaction buffer, and substrate, and can detect activity of any Gaussia luciferases or derivatives using coelenterazine as substrate.

Requirements

• Gaussia luciferase
• Luminometer or other luminescence detection instrument (e.g., Thermo Scientific Varioskan LUX Microplate Reader)

Applications

• Promoter studies for cis-regulatory and trans-acting factor analysis
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays for off-target effect studies
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

The Gaussia-Dura gene used in Thermo Scientific Gaussia-Dura Luc Vectors is derived from the wild-type Gaussia gene and provides equally bright but more stable light output.
The Glow Assay Kit reagent yields glow-type kinetics with lower intensity but longer duration, allowing higher sample throughput. This prolonged luminescence signal does not require reagent injectors and is ideal for luminometers without injectors or batch processing applications.

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