Thermo Fisher Scientific Pierce Cypridina Luciferase Glow Assay Kit, 100 Reaction Kit

Thermo Scientific™ Pierce™ Cypridina Luciferase Glow Assay Kit

The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

주요 특징

• Sensitive — Highly sensitive detection of Cypridina luciferase activity
• Secreted luciferase — Enables real-time assays and kinetic studies without destroying cells
• Stable — Reagents provide increased signal stability compared to flash assays
• Simple — Does not require a luminometer with injectors
• Compatible — Compatible with other Cypridina or Vargula luciferases
• Automation-friendly — Suitable for high throughput assays
• Convenient — Contains a universal cell lysis buffer and optimized glow assay reagent
• Safe — Non-radioactive assay system

제품 설명

This kit contains reagents for measuring Cypridina luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, it provides a highly sensitive bioluminescent reporter system for secreted or intracellular luciferase detection.

The light output generated by the luciferase reaction correlates with the amount of luciferase protein produced, which reflects promoter activity. The kit detects any Cypridina luciferases or derivatives that use Vargulin as a substrate.

구성품

• Cell lysis buffer
• Reaction buffer
• Substrate

필요 장비

• Cypridina luciferase
• Luminometer or compatible instrument (e.g., Thermo Scientific Luminoskan Ascent, Varioskan Flash Microplate Readers)

응용 분야

• Promoter studies for cis- and trans-regulatory analysis
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays for off-target analysis
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

기술 정보

Cypridina luciferase is a 61 kDa protein from the marine ostracod Cypridina noctiluca, secreted into cell culture media for live cell monitoring. The gene in Thermo Scientific Cypridina Luc Vectors is optimized for mammalian expression. The glow reagent yields glow-type kinetics suitable for luminometers without injectors or batch processing.

사양

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