Thermo Fisher Scientific O-linked N-acetylglucosamine (O-GlcNAc) Monoclonal Antibody (RL2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 125 publications 125 publications

Immunohistochemistry (IHC)

-

View 14 publications 14 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 6 publications 6 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 16 publications 16 publications

ChIP assay (ChIP)

Assay-dependent

View 4 publications 4 publications

Functional Assay (Functional)

-

View 1 publication 1 publication

Dot blot (DB)

1/800

-

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Chemical

Published species

Amphibian, Bovine, Chemical, Fish, Fruit fly, Hamster, Human, Invertebrate, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

RL2

Immunogen

Pore complex-lamina fraction purified from rat liver nuclear envelopes.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_326364

Product Specific Information

MA1-072 detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, virus and rat tissues.

MA1-072 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes up to eight different proteins from the NPC of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC. Immunofluorescence staining of the NPC with MA1-072 primarily labels NPC O-linked glycoproteins and has been successfully used on a wide variety of mammalian cells. Labeling occurs at the NPC, with most of the labeling at the cytoplasmic and/or nucleoplasmic margins, as well as within the nucleus.

The MA1-072 immunogen is pore complex-lamina fraction purified from rat liver nuclear envelopes.

Target Information

O-linked N-acetylglucosamine (O-GlcNAc) is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine or threonine residues. Unlike other protein glycosylations, O-GlcNAc modifications occur within the nucleus and cytoplasm. They are found on many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins. O-GlcNAc glycosylations are thought to obscure phosphorylation sites, counteracting phosphorylation-dependent signaling pathways and protein interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.