Thermo Fisher Scientific COX2 Polyclonal Antibody
COX2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 사용 가능. 인간 및 생쥐 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 0.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of rat COX-2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2549836 |
Product Specific Information
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
- After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
- Suggested positive controls: colon, lung, or breast carcinoma tissues.
Target Information
COX2 converts arachidonate to prostaglandin H2 (PGH2), a key step in prostanoid synthesis, including inflammatory prostaglandins.
This process involves two reactions:
- Cyclooxygenase (COX) reaction converting arachidonate to prostaglandin G2 (PGG2)
- Peroxidase reaction reducing PGG2 to PGH2
COX2 is constitutively expressed in tissues such as endothelium, kidney, and brain, and up-regulated under pathological conditions like cancer and inflammation.
Up-regulation is associated with increased cell adhesion, phenotypic changes, resistance to apoptosis, and tumor angiogenesis.
In cancer cells, COX2 promotes prostaglandin E2 (PGE2) production, influencing motility, proliferation, and apoptosis resistance.
COX2 is naturally inhibited by calcitriol (active Vitamin D) and is a known target of NSAIDs such as aspirin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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